City University New York Graduate Center

City University New York Graduate Center, Institute for Macromolecular Assemblies, CUNY, Biology Department, College of Staten Island, CUNY, USA. Websites: The City University of New York (http://www2.cuny.edu/) The College of Staten Island of the City University of New York (http://www.csi.cuny.edu/) The City University of New York (CUNY; http://www2.cuny.edu/) includes 11 senior colleges, seven community colleges, The Macaulay Honors College, the Graduate Center, the CUNY Graduate School of Journalism, the CUNY School of Law, the CUNY School of Professional Studies, and the CUNY School of Public Health, located throughout the city’s five boroughs. Each of the senior colleges offers a distinctive history, as well as a rigorous baccalaureate degree program and enriching campus experience. The CUNY Graduate Center offers more than 30 doctoral programs. CUNY School of Public Health, a collaboration of four CUNY institutions, offers master’s and doctoral degrees. The CUNY School of Law is recognized as the nation’s premier public-interest law school. CUNY is the nation’s largest urban public university. CUNY has 14 colleges offering more than 800 graduate degree programs, over 100 research centers and 21 world-class libraries. The blend of culture and social energy makes CUNY the greatest public university system in the greatest city in the world.  Serving more than 270,000 students in credit-bearing programs, and another more than 200,000 continuing and professional education students, the University confers 35,000 degree each year. The College of Staten Island of the City University of New York (http://www.csi.cuny.edu/) is one of the 11 CUNY senior colleges and is the only institution of public higher education in the borough of Staten Island. As of spring 2016, the college enrolled over 14,000 undergraduate students and more than 500 graduate students. The College of Staten Island offer Doctoral programs, Advanced Certificate programs, and Master’s programs, as well as Bachelor’s and Associate’s degrees. More specifically, the College offers over 87 undergraduate programs, and more than 27 graduate programs. For both research and educational purpose and activities, most of the faculty members at the College of Staten Island are also faculty members the CUNY Graduate Center. The faculty members that affiliated to the Graduate Center supervise PhD students and offer graduate courses. Therefore, the College of Staten Island is one of the research active colleges in CUNY. The College of Staten Island has provided research support for every researcher in the College. For example, the Co-Director’s laboratory space consists of 2 rooms in Biology Department. The laboratory has desk and bench space for a total of 4 researchers and all necessary facilities to conduct top-level research. Adjacent to the applicant’s laboratory is a cold room, warm room, autoclave, high level radiation facility, shop, and darkroom. The applicant’s laboratory contains: Applied Biosystems 7500 sequence detection system; Bio-Rad PDS-1000 Particle Bombardment apparatus; Turner 20/20 luminometer; 3 benchtop orbital air shakers/incubators; several microfuges; benchtop refridgerated low to high speed 1 liter capacity all-purpose centrifuge with 4 rotors; speed vac; laminar air flow hood; incubators; UV/VIS spectrophotometer with printer; ultrapure water purification system; several electrophoresis apparatus for protein, DNA, and RNA separations; Robbins Hybridization Incubator; bacteria incubator; 2 MJ DNA engine thermal cyclers; transilluminators and Polaroid photographic systems; image documentation system; -80 C freezer; gel drier; CO2 tissue culture incubator; inverted and upright microscopes with phase contrast and epiflourescence; UV cross-linker; balances; and sequence analysis software. The College also provides major equipment and facilities as shown below to conduct the proposed project. Additional shared equipment in Biology, Neuroscience, and Chemistry includes: Analytical HPLC (several Agilent 1100 and 1260 systems); LC-MS (Agilent 1100 series HPLC and LC/MSD Trap), Bruker Microflex MALDI-TOF spectrometry; Beckman LS5000TA scintillation counter; Picker X-ray film autoprocessor; Molecular Dynamics Storm 860 phosphoimager; Applied Biosystem Oligonucleotide Synthesizer; Beckman spectrophotometers including DV650 UV/VIS spectrophotometer; gamma counter; New Brunswick large floor model air incubator/shaker; Pharmacia/LKB analytical protein purification systems; and a Millipore/Waters 650E protein purification system. Beckman L8-80 ultracentrifuge and rotors; Sorvall RC5-C+ superspeed centrifuge and rotors; Beckman tabletop ultracentrifuge and rotors; additional -80 C freezers; PDI soft laser scanning densitometer; a Bio-Rad Model 550 microplate reader and Dynatech Ultrawash II microplate washer; a Savant SC100 speed vac concentrator with refrigerated vapor trap; a Savant SG200 gel dryer; and a Virtis 3L lyophilizer. Researchers have free access to: 200-800 MHz NMR; FTIR spectrometer; differential scanning calorimeter; FT-ESR; circular dichroism spectrometer; Flame Atomic Absorption Spectrophotometry instrumentation; Electron Microscopy and Confocal Facility: Transmission electron microscope is a Philips CM100 with an AMT digital camera connected to a PhotoPro 2000 printer with ancillary equipment including ultramicrotomes and knife makers. The SEM is an Amray 1910 field emission SEM. The confocal facility contains a Leica CLSM system attached to a Leica inverted microscope with a Leica image analysis system, Focus Graphics Imagecoder, and Sony Image Printer. The College also provides networking, computing and image processing system for the proposed research.